摘要:Graphical abstractDisplay OmittedHighlights•These methodologies have three key benefits.•Simultaneous determination of three endocrine disrupting phenol derivatives.•High sensitivity - detection by fluorescence detector.•Simply, short and not costly determination in blood and milk.Abstract•The aim of this work was to develop the methods of determination for phenol derivatives: bisphenol A (BPA), 4-tert-octylphenol (OP) and 4-nonylphenol (NP), in the whole body of herringClupea harengusand in physiological fluids of the Baltic grey sealHalichoerus grypus grypus(blood and milk).•Methods were based on liquid chromatography coupled with a fluorescence detector (HPLC-FL).•These methods were satisfactorily validated, each showing good recovery (>80%) and precision (RSD < 15%). Regarding the limit of quantification (LOQ), this was established at <2 ng g−1for herring, <0.07 ng cm-3for blood and <0.1 ng cm−3for milk.