期刊名称：Beni-Suef University Journal of Basic and Applied Sciences
印刷版ISSN：2314-8535
电子版ISSN：2314-8543
出版年度：2018
卷号：7
期号：4
页码：505-510
DOI：10.1016/j.bjbas.2018.06.001
语种：English
出版社：Elsevier
摘要：Highlights•Bioherbicidal activity, isolation and purification of the secondary metabolites produced by C1136 strain.•Structural elucidation of the active component.•Host range test and antimycotic activities of the active component.•The purified compound does not have any adverse effect on the germination, root length, shoot length of cowpea seedlings.AbstractThe use of synthetic herbicides poses a serious threat to environment, health and food safety. The development of safe and effective bioherbicides for selective control of weeds is thus the primary concern in crop production around the world. Efforts are being made to investigate the use of a safe, ecofriendly and highly selective herbicides from biological origin. Coumarin and its derivatives are natural compounds renowned for their phytotoxic potential.Mellein, a dihydroisocoumarin designated as (R)-8-hydroxy-3-methylisochroman-1-one was isolated as an active metabolite from the broth of a new strain (this strain is one of the best among phytopathogenic strains collected) ofLasiodiplodia pseudotheobromae. The structural characterization of the compound was achieved by using combined spectroscopic analysis i.e. 1D and 2D NMR and LC–MS. The host range study was carried-out against plants fromCucurbitaceae,SolanaceaeandLeguminosae,Poaceae and Valerianaceaefamilies, the isolated phytotoxic metabolite fromLasiodiplodia pseudotheobromaeat a concentration 10 µg/µl showed selective inhibition at 56%–66% against the latter two families only when inoculated.Hence, purified compound fromLasiodiplodia pseudotheobromaeis recommended for large scale production of bioherbicides for the management of weeds in conventional farming to improve yield and enhance food security.
关键词：Bioherbicides;Lasiodiplodia pseudotheobromae;(;R;)-8-Hydroxy-3-methylisochroman-1-one and weed
