Methods: This descriptive study was conducted on 120 healthcare employees in Tehran east health center in 2013. Participants were selected through the two-stage cluster sampling. Data were collected via a researcher-made satisfaction assessment questionnaire which included subjects about the content, implementation method and the process of implementing e-learning program. The collected data were analyzed using descriptive statistics, Chi-square test, Mann-Whitney, and Pearson correlation coefficient.

Results: The results showed that the highest personnel satisfaction score was in teachers’ accountability in practical work (22.24±1.80 out of 25) and the lowest satisfaction scores were related to how to complete the questionnaire electronically (17.28±2.32), and using good examples and illustrations for better understanding of the content (17.43±1.90). Satisfaction with other categories were respectively related to being forced to think about the content, receiving database confirmation after registering personal information, the content (web-based brochures, images or videos), online group discussions in chat rooms, and appropriateness of the content of LMS meetings with professional needs. Factors such as education (p=0.001), job (p=0.001) and employment status (p=0.004) had impacts on personnel’s general satisfaction with participating in the e-learning course.

Conclusion: The results showed significant personnel`s satisfaction with the quality of web-based learning in teaching preventive behaviors of hepatitis B virus infection. Given the fairly lower satisfaction rates in some categories of the implementation method and content of the e-learning program, it is necessary to take these into account when designing such programs in order to improve their quality.