摘要：AbstractPoint‐of‐care devices that are inexpensive, disposable, and environmentally friendly are becoming increasingly predominant in the field of biosensing and biodiagnostics. Here, microfluidics is a suitable option to endow portability and minimal reagent and material consumption. Nanocellulose is introduced to manufacture microfluidic channels and as a storage and immobilization compartment of glucose oxidase. Improved enzymatic activity retention is demonstrated in a simple and disposable point‐of‐care diagnostic unit that is able to detect glucose from fluid matrices at 0.1 × 10−3mconcentration and in less than 10 min. It is concluded that the patterning and fluidic technologies that are possible with nanocellulose enable easily scalable multianalyte designs.Renewable and environmental friendly nanocelluloseacts as a material for microfluidic channels and as storage for glucose oxidase enzyme immobilization for improved activity retention. A simple, enzyme based, disposable point‐of‐care diagnostics approach is designed for the detection of glucose at 0.1 × 10−3mconcentration in less than 10 min. The patterning and fluidic technologies allow concept scale‐up to multianalyte designs.
