摘要：Research on effective micro-organisms (EM) mainly focuses on their effect on crop yield and crop quality. On the other hand, knowledge on the effect of EM on metabolic processes that take place in plants at the cellular level is not sufficiently systematised. The subject of this study was to evaluate the effect of an EM preparation on two oxidative stress parameters, i.e. free proline and malondialdehyde (MDA) contents in the green parts of sweet basil Ocimum basilicum L. var. Piccolino grown in pots. The concentration of free proline was determined by the ninhydrin reaction and the malondialdehyde concentration based on the reaction with thiobarbituric acid. Analysis of variance for selected factors showed a significant effect of interaction both EM and time on the decrease of proline and MDA concentration. Effect of singular factor i.e. EM has shown a favourable influence of the preparation on the oxidative stress parameters in sweet basil by lowering the concentration of proline and significant slowing down the process of lipid peroxidation in the plant tissues. EM can be used in crop growing as a preparation to facilitate the adaptation of plants to changing climatic and habitat conditions.
