出版社：Japan Science and Technology Information Aggregator, Electronic
摘要：Dynamic binding capacity (DBC) of model proteins in ion-exchange chromatography was analyzed based on a simplified pore diffusion model in order to develop a simple method for designing a capture chromatography process. A dimensionless parameter, F0＝u0dp2/(ZDm) was derived from the pore diffusion model, where u0 is the superficial velocity, dp is the particle diameter, Z is the column bed height, and Dm is the molecular diffusion coefficient. Breakthrough curves of model proteins (lysozyme and immunoglobulin G) were measured for cation exchange chromatography columns of two different particle sizes (dp＝34 and 90 μm) at various temperatures (8, 13, 18, 23 and 28℃). The efficiency E＝DBC/SBC values calculated from the experimental data were well correlated to F0 (SBC＝static binding capacity). The E-F0 curve was found to be useful for designing efficient capture processes of proteins.
