摘要：The hotspot is to evaluate and improve Songnen grassland with alkali-saline becoming more severe, but little is known about soil microbial diversity in alkali-saline grassland. To reveal the influence of alkali-saline on soil microbial diversity in Songnen grassland, we measured and analyzed soil pH and conductivity, soil microbial counts, biomass carbon, and genetic diversity in different degree of alkali-saline grassland. The results showed that soil microbial counts and biomass carbon reduced along with the alkali-saline of grassland. They had a significant negative correlation with soil pH and conductivity. Soil microbial community composition reduced 15.79% in response to the alkali-saline of grassland. Only Staphylococcus aureus appeared. For further investigated by phylogentic tree, they belong to Bacilli. Nevertheless, Soil microbial genetic diversity had insignificant change. To sum up, Soil microbial quantity were sensitive to alkali-saline of grassland, which could be used as an index to define the alkali-saline of grassland. But Soil microbial genetic diversity and community composition had no significant changes.
