摘要:This work investigated the improvement of biodesulfurization (BDS) of bunker oil via different approaches, such as adding surfactant, adding solvent and using ultrasonic irradiation pre-treatment. The effectiveness of these approaches was evaluated and compared. Experiments were also carried out on separation of oil/aqueous phase after BDS by adding the HS-P01®. The results showed that after adding the surfactant, solvent or ultrasonic pretreatment, the BDS efficiency of bunker oil was improved evidently after bio-treatment. About 10-20% of sulphur was removed from the samples with different approaches.Ultrasonic irradiation pre-treatment was better as approach for improved BDS of bunker oil, in comparison to adding surfactants and solvents. The oil, aqueous medium and a biomass could be separated by adding 1% HS-P01®. 78.6% oil was recovered in the best run which was pre-treated by ultrasonic irradiation. The recovery of treated oil was less effective when surfactants were added in during the BDS process.