摘要：AbstractBrassica rapaL. is cultivated globally and consumed in many areas worldwide. Using the transgenicAgrobacterium-mediated transformation method, which is a reproducible and efficient technique, genes can be transferred into variousB. rapaspecies. This review summarizes the processes involved inAgrobacterium-mediated transformation ofB. rapa, including surface seed sterilization, co-cultivation withA. tumefaciens, induction of callus/shoot/root formation, and confirmation of transgenic plants. In addition, factors such as theAgrobacteriumstrain, plant genotype, explant age, transformation efficiency of the hybrid or inbred line, and the concentrations of N6-benzyl amino purine and naphthalene acetic acid, are discussed. And this review shows clearly how to do it, what to do, and what not to do in the transgenicAgrobacterium-mediated inBrassica rapa. The information presented here lays the foundation for a simple and efficient method that resolves existing problems and improves overall transgenicB. rapaproduction, thereby benefiting both basic and applied research.
