摘要：To determine the composition of the bacterial communities associated with cyanobacteria in thefreshwater ponds of Channa argus in intensive culture in the late culture period, we examined thebacterial community of non-cyanobacterial blooms freshwater ponds (C) and cyanobacterial bloomsfreshwater ponds (CB) using PCR-denaturing gradient gel electrophoresis (PCR-DGGE) and 16SrDNA clone library. DGGE profiles showed that Microcystis sp. (JX391871.1), Leptospira sp. (JX067673.1)and Verrucomicrobium sp. (FN668203.2) existed only in CB, whereas Sphingomonas sp. (AB681542.1)and Paenibacillus sp. (JQ659563.1) were the C-specific bacteria. The 16S rDNA clone libraries ofpond water showed that Actinobacteria (15 OTUs, 31.9%), Bacteroidetes (13 OTUs, 28.7%),Fibrobacteres (7 OTUs, 14.9%) and Proteobacteria (5 OTUs, 10.0%) were the dominant groups in C;the four dominant groups in CB were Bacteroidetes (17 OTUs, 34.7%), Actinobacteria (9 OTUs,18.4%), Fibrobacteres (6 OTUs, 12.2%) and Proteobacteria (8 OTUs, 16.2%). In the clone library,distribution of sequences belonging to Fibrobacter in CB (22.2% of the 99 clones) was twice as thatof C (11.1% of the 99 clones). In both DGGE map and 16S rDNA clone library the Firmicutes group(JQ659563.1 in DGGE and 3 OTUs in C library) existed only in C. These results suggested that thecomposition of bacterial communities changed with the cyanobacterial blooms in Channa argus intensiveculture freshwater ponds, and whether the change of the Firmicutes-to-Bacteroidetes ratio could beused to predict cyanobacterial blooms in these types of ponds required further validation.
