摘要：Biological treatment of pyridine wastewater is difficult because of it’s poor biodegradability. In order todeal with the problem, this study applied glucose as co-metabolism growth substrate to build cometabolism system for biological degradation of pyridine in Sequencing Batch Biofilm Reactor (SBBR).The study investigated the effect of dissolved oxygen (DO) on the system building. The result showedthat DO had significant influence on the establishment of the system. When the pyridine loading was 0.5 kgCOD m-3 d-1 and the glucose loading rate was 1.0 kg COD m-3 d-1, the most suitable DO was 3.5 mg/L.After 70 days operation, the pyridine removal efficiency reached stable, which was 83.6%. Meanwhile,under the condition that DO was 3.5 mg/L, pyridine loading had an effect on pyridine removal efficiency.The favourable pyridine loading was 0.5 kg COD m-3 d-1. PCR-DGGE illustrated that the microbialcommunity varied under different DO concentrations. Compared with seed sludge, the similarity were50.2%, 44.1%, 35.8% and 62.9% respectively, when DO were 0.5, 2.0, 3.5 and 5.0 mg/L. Fouriertransforms infrared spectroscopy (FTIR) results showed that primary amides and carboxylic acidsgenerated in effluent as intermediate products during pyridine degradation process.
