摘要：Pseudomonas aeruginosa is a Gram negative and motile bacterium with different virulence factors.Nowadays, the reports from worldwide illustrated antibiotic resistance of this opportunistic bacterium.Hence, the present study tried to isolate clinical and environmental samples and compare the antibioticsusceptibility as well as enzyme profiles of the isolates. For this purpose, 100 specimens fromenvironmental sources and clinical sources have been collected. The samples were cultivated oncetrimide agar and blood agar, then the plates were incubated at 35°C for 24-48 hrs. The suspectedcolonies were purified and preliminarily characterized using Gram reaction, catalase, oxidase andmotility test. Then they were identified using Api 20 NE kits. Afterward, antimicrobial susceptibility ofthe isolates was evaluated using Kirby-Bauer test, and enzyme profile were checked using API ZYMkit. The results obtained from this study indicated that out of 50 specimens, 10 isolates belonged to theenvironmental samples and 13 belonged to the clinical one. In addition, the highest isolation rate ofclinical samples was belonging to the wound specimens. Furthermore, the results indicated that thereis no difference between the enzyme profiles of the isolates, but regarding to the antibiotic susceptibility,the environmental samples were more sensitive than the clinical one.
