摘要：Lead (Pb) is one of the most common heavy metals in nature which has an adverse effect on aquaticecosystems and affects animal health. Animal survival depends on its reproductive ability. To analysethe influence of Pb on the male testis tissues in the spot frog (Pelophylax nigromaculata), the maleadult individuals were exposed to Pb (10, 100 and 1000 µg/L) in water for 30 days. We observed thespermatic histopathologic structure and detected the expression and location of androgen receptors(AR) on the germ cells by immunohistochemical method. Compared to the control, the testishistopathologic structure and the expression of AR at Pb treatment were different. At relatively higherconcentration (100 and 1000 µg/L), the changes of spermatic pathological mainly included germ cellnecrosis, congestion and fibrosis congestion. The expressions of AR on the gonad cells wereaffected by Pb. With the increase of Pb concentration, AR expressions were decreased.
