摘要：Carbendazim (MBC) is one of the most widely used agricultural fungicides worldwide, but this chemicaladversely affects animal health and ecosystem function. As such, effective methods for remediating MBC-contaminated soils must be developed. In this study, a novel MBC-degrading bacterium, namely, djl-5B, wasisolated from MBC-treated soil. Based on its phenotype features as well as physiological, biochemical, andphylogenetic characteristics, the strain was identified as Pseudomonas sp. The optimal degradation conditionfor strain djl-5B was determined to be at 30°C and pH 7.5 with an inoculum size of 7%. Inoculation of straindjl-5B to MBC-contaminated soil significantly decreases the degradation half-life of MBC from 14.15 d to3.06 d in non-sterile soil and from 24.75 d to 3.30 d in sterile soil. Results of high-pressure liquidchromatography-mass spectrometry analysis revealed that the degradation product of strain djl-5B contains2-aminobenzimidazole, 2-hydroxybenzimidazole and benzimidazole. The newly isolated strain effectivelydegrades MBC and thus exhibits potential for environmental rehabilitation. This study provides basicinformation and technical support for future utilization of strain djl-5B as an ecofriendly bioremediation agent.
