摘要：The genus Rhodococcus is a Gram positive and non motile bacterium with different capability. Nowadaysscientists are using the organism for the production of their antimetabolite products. Therefore, in the presentstudy, the genus was isolated from the soil of different parts around the manganese mine in Qom city, Iran.The isolates were characterized using biochemical and antimicrobial test. Antimicrobial properties wereevaluated using pathogenic bacteria, including: Staphylococcus aureus (ATCC 25923), Escherichia coli(ATCC8739), Pseudomonas aeruginosa (ATCC 27853), Salmonella typhimurium (ATCC14028), Shigelladysenteriae (PTCC 1188), Klebsiella pneumophila (ATCC 700603), Candida albicans (ATCC 10231), Bacillussubtilis (PTCC1715), Bacillus cereus (PTCC 1015), Listeria monocytogenes ( PTCC1295), Enterococcusfaecalis (ATCC51299), Rhodococcus erythropolis (PTCC1767), Corynebacterium glutamicum (PTCC 1532).Furthermore, antibacterial compounds from culture supernatant were extracted using different solvents andthe extracts were analysed using GC-MS. The results indicated that 9 of the isolates correctly belonged tothe genus Rhodococcus. In addition, the results exhibited potential antibacterial activity againstStaphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC8739), with average zone inhibition of 8and 25 mm respectively, but it had no effect on the other organisms. On the other hand the chromatogramshowed nine special peaks and the identified compounds were: cyclopenthasiloxane, azulene, 2,7 octadiene,docosane, cyclohexasiloxane, bis (3,5,5 trimethyhexyl, tetraedacane-2 methyl, cycloheptasiloxane andheptadecanol. Phylogenetic analysis showed that the isolated strain from soil around manganese mine inQom city, Iran formed a distinct subclade within the genus Rhodococcus with R. rhodochrous.
