摘要：The aim of the research work was decolourisation of Remazol Brilliant Blue R (an anthraquinone dye) andguaiacol by free cells and immobilized strains of Bacillus sp. D1023, D1032 and D1033 which were isolatedfrom field soil of Ambala College of Engineering and Applied Research. Comparison between free cells andimmobilized cells was observed using 100 mg/L of Remazol Brilliant Blue R and efficiency of beads wasobserved by reutilization of beads using same concentration of dye. The selected strains D1023, D1032 andD1033 were able to decolourise RBBR dye up to 81.23%, 73.14% and 75.44% by free cells and 94.54%,84.45% and 89.50% by immobilized cells respectively. These strains are also utilized to treat Guaiacol andcorresponding colour change has been observed for free and immobilized strains. To increase thedecolourisation process 2×SG medium was used which increases the spore production in culture.
