摘要：The bacterial pigments are secondary metabolites that help the producer to survive in differentenvironments. The bacterial pigments have shown different properties such as antitumor, anticancerand antimicrobial effects. The present study was conducted to isolate soil origin Actinomycetes and toevaluate their antimicrobial pigments. In total, eighty seven soil samples were collected from differentareas in Kazeroun city, Iran. The samples were serially diluted (10 -1 to 10 -7 ) and 0.1 mL of each dilutionwas streaked on SCA and ISP2 media and incubated at 30C° for 5 days. Then pigmented Actinomyceteswere selected and subjected for propagation and pigment extraction using different solvents. Tocontinue the experiments, the antimicrobial property of each pigment was evaluated againstStaphylococcus aureus, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, Shigelladysentriae, Citrobacter ferundii, Klebsiella peumoniae, Serratia marcecens, Aspergillus sp. andCandida albicans. Furthermore, identification of the promising strain was verified by 16srDNA genesequencing method and finally different groups in the pigment structure were determined by TLC andreagent spray tests. The results obtained indicated that out of all the isolated Actinomycetes strains,Streptomyces coeruleorubidus could produce pigment with antimicrobial property. Of all themicroorganisms tested Staphylococcus aureus was more sensitive and Aspergillus sp., Candidaalbicans and E. coli were more resistant. Our finding concerning to structure of the pigment illustratedexistence of alcohols, phenols and steroid groups in the pigment. Overall, soil origin Actinomycetescould produce pigments with antimicrobial property. Therefore, Actinomycetes pigments must beconsidered a special remedy for investigation in order to eliminate occurrence of antibiotic resistantmicroorganisms.
