摘要：The purpose of this study was the evaluation of soil origin Brevibacillus for production of bioactive compounds.Nowadays bioactive compounds are considered the sources of new remedy in order to eliminate antibiotic-resistant bacteria. To perform the study, two strains of Brevibacillus with potent activity for production ofbioactive compounds were isolated from 121 soil samples. Phenotypic and 16SrRNA sequencing identificationof the isolates recognized them as Brevibacillus reuszeri (B12 bioactive compound producer) and Brevibacillusformosus (B22 bioactive compound producer). The bioactive compounds produced by the bacteria werepartially purified and characterized based on their antimicrobial activities at various pHs and temperaturesand proteinase K. Then, activity of the bioactive compounds was assessed against different pathogenicmicroorganisms. The results obtained indicated that activity of the compounds when treated with proteinaseK was inhibited. The optimum pH for both the isolates was 7 and potent activity of the bioactive compoundB12 was observed at 35°C while for B22 was at 40°C. Although, Bacillus cereus, Staphylococcua aureus,E. coli and Salmonella typhi were sensitive, Pseudomonas aeruginosa and fungi (Candida albicans andAspergillus niger) were resistant to the both bioactive compounds. Therefore, Brevibacillus with potentactivity for production of antimicrobial metabolites, and fast growth character may be considered as a goodprobiotic agent for commercial industries.
