摘要：The growth characteristics of seven strains isolated from oil contaminated soil, as well as their respectivedegradation efficiency for various hydrocarbons were investigated. Factors that can impact biological oildegradation efficiency were revealed in a series of experiments. The results indicate that isolated strainscould rapidly degrade crude oil, showing high activity in the first 13 h of bioremediation. These strains couldgrow in paraffin wax, which indicates that these strains could degrade long chain hydrocarbons. Some ofthem (SY 22 , SY 23 , SY 24 , SY 42, SY 43 ) were able to use short chain hydrocarbons and aromatic hydrocarbonsas substrate, so these five strains are the preferred ones for the bioremediation of oil contaminated soil.Suitable pH for the growth of these five strains was in the range from 7 to 9. NH 4 NO 3 and oil concentrationsshould range from 1000 mg/L to 1500 mg/L in order to achieve optimum conditions for petroleum hydrocarbondegradation. Adding organic matter such as starch and glucose accelerated oil and PAH degradation capabilityof the SY 22 , SY 42 and SY 23 strains. The presence of metal ions, such as Ni 2+ and Co 2+ in soil decreased thecrude oil degradation efficiency of these strains, while metal ions, such as Fe 2+ and Mn 2+ did not affect the oildegradation activities.
