摘要：Biosurfactants are surface active compounds which are produced by bacteria, fungi and yeasts. Most ofthem have different structures including: lipopeptides, glycolipids, polysaccharides, protein complexes, fattyacids and phospholipids. Nowadays, due to their useful properties, they have attracted attention of many.Therefore, the present study was conducted to isolate Gram positive bacteria capable of producingbiosurfactants from Mighan Wetland in Iran. Accordingly, the isolated microorganisms were evaluated usingoil spreading technique with different types of oil and haemolysis tests. The selected microorganisms weredetected by their ability to produce surfactants using TLC. The results indicated that out of seven differentisolated genera two were Gram positive, and they were characterized as Bacillus firmus and Staphylococcussp. On the other hand, oil spreading technique indicated that organisms are able to produce biosurfactants.In addition, extracted biosurfactant on TLC plates and applying ninhydrin reagent indicated the lipopeptidestructure of the biosurfactant by producing red spot. Hence, the present study illustrated that this area ofinvestigation could be a suitable place for isolation of microorganism with capability to produce biosurfactantsand it could be used for further study and applications.
