摘要:The biomethanated distillery spentwash is a nutrient rich liquid organic waste obtained from molasses baseddistillery industries after biomethanation process. The spentwash, being loaded with organic compoundscould bring remarkable changes on the biological properties of soils and thus influences the fertility of soilsignificantly. The effect of different levels and methods of spentwash application on soil microbial activitywas examined through a field experiment. The field experiment was conducted on groundnut (Arachishypogaea L.) as a test crop at Research and Development Cane Farm, The Salem Co-operative Sugar MillsLtd., Mohanur, Namakkal District. Application of distillery spentwash significantly increased the microbialpopulation viz., bacteria, fungi and actinomycetes in soil. The application of spentwash at the rate of 120 m 3 /ha plus NP fertilizers recorded the highest microbial population. The continuous application of split doseswas found to be better than one time application of spentwash in promoting the microbial activities.