摘要:The major purpose of this study was isolation, identification and characterization ofCampylobacter spp. from environmental samples viz., domestic animals (cow, sheep,horses), poultry, sewage and river water in north Iran. Campylobacter spp. were isolatedusing pret-KB method and then identified by phenotyping tests. Finally, the identificationof strain was verified by 16S rRNA gene sequencing. In general, 64 strains ofcampylobacters were isolated from all the sources. Out of all isolates 48 strains werecatalase positive and 16 strains were catalase negative. The highest isolation rate ofCampylobacter spp. was recorded from river water (36.92%) followed by poultry(34.88%), cow (28.57%), horses (20%) and sheep (9%), while lowest isolation ratewas recorded from sewage (7.4%). Overall, according to these data Campylobacterspp. exist with high frequency in north Iran. In addition, this bacterium was isolatedfrom all sources (river water, sewage, domestic animals and poultry). Hence, based onforegoing evidence environments in north of Iran are vehicles of Campylobacter spp.and, therefore, the people who live in this area must respect to the personal hygiene inorder to avoid from Campylobacter infection.