摘要：AbstractGenome editing offers great advantages in identifying gene function and generating agronomically important mutations in crops. Here, we report that the genome of cabbage (Brassica oleraceavar.capitata), an important cruciferous vegetable, can also be precisely edited by a CRISPR/Cas9 system stacked with multiple single-guide RNA-expressing cassettes. When the phytoene desaturaseBoPDSgene was used as the target gene, an albino-phenotype transgenic shoot in T0Basta-resistant lines was observed, and 37.5% of the transgenic cabbage shoots carriedBoPDSgene mutations as a result of nucleotide deletions at the expected position. Moreover, mutations were detected in sites with the same target sequence in geneBol016089which is paralogous to theBoPDSgene. Our results show that the CRISPR/ Cas9 system is a powerful tool for cabbage variety improvement by genome editing.
