摘要：AbstractPlants with crassulacean acid metabolism (CAM) generally utilize water 20%–80% more efficiently than non-CAM plants. The whole genomes of several CAM plants have been sequenced or are being sequenced. For effective genome characterization and genome editing of CAM plants, an efficient transformation system is essential. In this study, we developed anAgrobacterium-mediated transformation protocol forKalanchoe laxiflora,an obligate CAM plant,by optimizing several factors affecting the transformation efficiency.Agrobacteriumstrains AGL1, C58, EHA105, and GV3101 were all suitable forK. laxifloratransformation. Fifty-nine percent of the leaf explants yielded kanamycin-resistant and GUS-positive shoots. Polymerase chain reaction and quantitative real-time PCR (qRT-PCR) usinggusA-, gusPlus-, nptII-andhpt-specific primers confirmed that the transgenes were integrated intoK. laxifloragenome and expressed. This efficient transformation system will allow effective functional characterization of genes through over- or down-expression, knockout, or genome editing.
