摘要：Reactive Red 195 (RR-195) is a sulphonated azo-dye used extensively in textile industries. The current study was carried out to investigate the decolourisation of RR-195 dye using a novel bacterial strain, in an attempt to overcome the environmental hazards caused due to the unopposed industrial laws and substandard waste management systems. The most promising bacterial strain isolated from textile effluent was identified as Enterococcus casseliflavus RDB_4 by 16s rRNA analysis. It showed complete decolourisation of 50 ppm dye in optimized M9 medium (pH 7) supplemented with 3% yeast extract, within 4 h at 35°C using inoculum size of 0.2 O.D530nm under static conditions. Moreover, it showed tolerance to high NaCl (1 to 6%) and dye (1000 ppm) concentrations in batch as well as continuous culture systems; thus making it ideal for industrial dye waste management. Furthermore, the biodegradation of RR-195 dye was confirmed by high-performance liquid chromatography and Fourier transform infrared spectroscopy analysis into nontoxic metabolites ensured by ecotoxicity studies.
关键词：Decolourisation;Degradation;Enterococcus asseliflavus;RDB_4;Reactive Red 195
