摘要：It is a major method to produce ginsenoside Rg3 using microbial transformation. Therefore the selection of efficient strains is the prime requirement. Based on the synergetic relationship, the microbial flora R2A43 was obtained from the soil of planting ginseng, which had a strong capability to transform ginsenoside Rg3 by the method of TLC and HPLC, the content of 20(S)-ginsenoside Rg3 was 2.32mg/g. High-throughput sequencing results showed that the dominant flora of Bacterium were Bacillus (93.53%) and Allobacillus (4.3%). The dominant flora of Fungus were Unclassified (56.53%), Penidiella (14.95%), Inonotus (3.23%), Malassezia (3.54%), Ophiosphaerella (3.04%), Gibberella (2.15%), Pleospora (2.12%), Pyrenoph- ora (1.81%), Filobasidium (1.46%), Kazachstania (1.46%), and Aspergillus (1.26%). The transformation pathway was Rb 1→Rd→Rg3. Compared with single strain, this study provides a new method and mentality for the microbial transformation on ginsenoside Rg3, and had the significance in the production of ginsenoside.
