摘要：Among many other plant diseases Chili Leaf Curl Disease (ChLCD) caused by Begomovirus complex is a crucial threat for chili worldwide. Chili Leaf Curl Virus (ChLCV) is monopartite s DNA Begomovirus having DNA-A which comprises of 2754 nucleotides having six ORFs and betasetallite of size 1.3kb. Genomic DNA of infected chili leaf samples was extracted by CTAB method, collected from different field areas of Faisalabad. Circular molecules from genomic DNA were amplified by using Rolling Circle Amplification (RCA). Three samples showed successful RCA which further used in PCR amplification. For amplification of full length Begomovirus from RCA product universal primers were used. Betasatellite component associated with ChLCD was amplified with the help of PCR by using full-length universal primers (β01 and β02). RF1LP analysis showed that single type of Begomovirus and diverse type of betasatellite molecules observed in infected chili samples.
