标题：IDENTIFICATION AND EXPRESSION ANALYSIS OF ACID INVERTASES GENE FAMILY IN ROOTS OF METALLICOLOUS AND NON-METALLICOLOUS POPULATIONS OF RUMEX JAPONICUS UNDER COPPER STRESS
摘要：Recent researches indicate that under heavy metal stress, metallicolous population (MP) of met- allophyte display a higher activity of acid invertases enzymes, which are rate-limiting in sucrose catabolism, than non-metallicolous population (NMP). Because most of these metallophytes are non-model plants for which the background genomic information is limited, it is unclear whether the higher activities of acid invertases are the result of higher expression of individual or total acid invertases gene family members. Based on high throughput RNA sequencing, in present study we identified eight acid invertases genes, including six cell wall invertase genes (RjcwINVl-6) and two vacuolar invertase genes (RjvINVl-2), from MP and NMP of Rumexja- ponicus, and determined their expression patterns under Cu stress. The seedlings of R. japonicus were exposed to control or 20 µM Cu~(2+) for 48 hours under hydroponic conditions. The transcript levels of RjcwINV2, RjcwINV5 and RjvINv1 in MP were significantly higher than them in NMP, while RjvINV2 only expressed in NMP. The results suggested that different members of the acid invertases gene family have varying expression patterns under Cu stress. Furthermore, the higher acid invertases activities of MP under Cu stress could be due in part to elevated expression of individual instead of total acid invertases genes.
