摘要:In this study, antioxidant activity was evaluated by selected mosses DPPH, β-carotene /Linoleic acid test, ABTS, and CUPRAC methods. This is the first time DNA damage protection effect of seasonal differences Fontinalis antipyretica Hedw. and Hypnum cupressiforme Hedw. The amount of total phenolic and flavonoids were high in F. antip-yretica methanol extracts in spring season. The amount of phenol- ics content and flavonoids were high in F. antip-yretica methanol extracts in spring season than others. F. antipyretica methanol extracts in winter showed better DPPH free-radical scavenging assay and β-caro- tene /Linoleic acid assay. The highest CUPRAC and ABTS antioxidant activity was seen in winter and spring season H. cupressiforme chloroform and methanol extracts, respectively. The protection against DNA damage activity of extracts were studied by agarose gel electrophoresis method. It was observed that spring season chloroform extract of H. cupressiforme completely disintegrate DNA in 1μM concentration.
关键词:Fontinalis antipyretica;Hypnum cupressiforme;Phytochemical screening;DPPH;CUPRAC;ABTS;pBR322 DNA