摘要:AbstractThis research studied the chemical constituents and antibacterial activity of essential oils from the areal parts ofMentha×piperitaL. essential oil. Essential oil was subjected to hydrodistillation for 4h using Clevenger apparatus, resulting in nineteen chemical constituents representing 100% of the essential oil, comprising menthol (36.02%), menthone (24.56%), menthyl acetate (8.95%), and menthofuran (6.88%); these are major components, and others are minor components. Essential oil shows significant antibacterial and antifungal activity than principle components. The essential oil shows significant antibacterial activity against human pathogenic micro-organisms. Further,Staphylococcus aureus(42.44±0.10mm),Micrococcus flavus(40.01±0.10mm), Bacillus subtilis(38.18±0.11mm),Staphylococcus epidermidis(35.14±0.08mm), andSalmonella enteritides(30.12±0.12mm) show the highest antibacterial activity against essential oils. Essential oils show significant antifungal activity againstAlternaria alternaria(38.16±0.10mm),Fusarium tabacinum(35.24±0.03mm),Penicillumspp. (34.10±0.02mm),Fusarium oxyporum(33.44±0.06mm), andAspergillus fumigates(30.08±0.08mm). The maximal and minimal inhibition concentration values are in the range of 10.22±0.17 to 38.16±0.10 and 0.50±0.03 to 10.0±0.14μg/ml, for yeast and fungi respectively. The present study on essential oils deriving from theMentha×piperitaL. species could be used in antimicrobial activity as a natural source.