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  • 标题:Molecular characterization of Bacillus, lactic acid bacteria and yeast as potential probiotic isolated from fermented food
  • 本地全文:下载
  • 作者:Hama Cissé ; Boureima Kagambèga ; Adama Sawadogo
  • 期刊名称:Scientific African
  • 印刷版ISSN:2468-2276
  • 出版年度:2019
  • 卷号:6
  • 页码:1-13
  • DOI:10.1016/j.sciaf.2019.e00175
  • 语种:English
  • 出版社:Elsevier
  • 摘要:AbstractSpontaneous fermentation or traditional method of preserving indigenous foods using several microorganisms, is frequently practiced in the marginal world. Fermented seeds and milk are mostly consumed in the form of food condiments and desserts in Africa, Asia and others parts of world. Our previous studies deal with the production of Bacteriocin-Like Inhibitory Substances byBacillusfrom fermented food and quality of fermented milk consumed in Burkina Faso. The rep-PCR and sequencing were used to characterize thirty eights strains isolated various fermented foods from Burkina Faso. Phylogenetic tree were constructed by the neighbour-joining method based on 16S or 26S rRNA genes sequences using MEGA X. Based on colonies characteristics and cells morphology, biochemical tests and gene sequencing, the isolates were identified asBacillus cereus sensu lato(13),Bacillus pumilusgroup (03) with one strain (LCG1) presumed LAB was identified asBacillus australimarisorBacillus pumilusby 16S rRNA sequencing,Enterococcus durans(03),Lactobacillus paracasei(03),Lactobacillus plantarum(04),Leuconostoc pseudomesenteroides(01),Saccharomyces cerevisiae(04),Kluyveromyces marxianus(01),Candida tropicalis(01),Pichia kudriavzevii(01),Clavispora lusitaniae(02),Rhodotorula mucilaginosa(01) and,Cyberlindnera fabianii(01). Several microorganisms with potential technological interest are housed in fermented foods from Burkina Faso. These microorganisms are responsible for the fermentation of food through their enzymatic activity, leading to production of fermented food with desirable organoleptic characteristics, improved food safety, the enrichment of nutrients and the promotion of health of consumers.
  • 关键词:KeywordsFermented foodsBiodiversityBacillusLactic acid bacteriaYeasts, Rep-PCR
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