期刊名称：Current Journal of Applied Science and Technology
印刷版ISSN：2457-1024
出版年度：2019
卷号：38
期号：2
页码：1-7
DOI：10.9734/cjast/2019/v38i230354
语种：English
出版社：Sciencedomain International
摘要：Aim: Canine Parvovirus (CPV) is an emerging and re-emerging virus of canines. The study was
undertaken to analyze VP2 gene of CPV in the isolates from dogs positive for CPV infection.
Methods: The rectal swabs were collected from dogs suspected of CPV and subjected to PCR and
nested PCR. The regions compared in the study were Punjab to represent north part of India and
Assam to represent north-eastern part of India. The sequence analysis of VP2 gene of CPV was
done using NCBI BLAST from the isolates which were positive for CPV by nested PCR. Further,
phylogenetic analysis was done to understand the prevailing antigenic type of CPV from northern
and north eastern part of India.
Results: The sequence analysis revealed that all the sequences of VP2 gene from the samples had
98-99% homology with Canine Parvovirus and phylogenetic analysis revealed that CPV 2a antigenic
type is circulating in both the regions selected in the study.
Conclusions: The present study revealed that CPV 2a is circulating in the regions of Punjab and
Assam part of India. Further sequence analysis of VP2 gene from more number of field isolates can
throw better light on the prevailing antigenic type of CPV in various parts of India.
