期刊名称：Current Journal of Applied Science and Technology
印刷版ISSN：2457-1024
出版年度：2019
卷号：38
期号：2
页码：1-15
DOI：10.9734/cjast/2019/v38i230357
语种：English
出版社：Sciencedomain International
摘要：Aims: The aim of this study was to explore the metabolic profile of endophytic fungi, Aspergillus
terreus (ENF12) isolated from mulberry leaf and evaluate their antifungal efficacy against
Macrophomina phaseolina causing charcoal rot disease of mulberry.
Study Design: Completely Randomized Block Design.
Place and Duration of Study: Department of Plant Pathology, Tamil Nadu Agricultural University,
Coimbatore, Tamil Nadu during August 2018 to June 2019.
Methodology: The isolation was done by serial dilution and plating method. The culture was
identified by utilizing the morphological, microscopical and taxonomical keys as A. terreus. The
macroscopic observations such as colony growth, color, texture, pigmentation, growth rate and
microscopic observations viz. hyphal and conidial characters were studied. The molecular
confirmation was done by DNA sequencing using ITS 1 and ITS 4 primers. The extracellular
secondary metabolites were extracted from A. terreus and screened against M. phaseolina by agar
well method. The composition of the metabolites was analyzed through GC-MS.
Results: The septate and hyaline mycelium along with aleurioconidia was observed under
microscope and based on the DNA sequencing, the ENF12 isolate was identified as A. terreus. The
major compounds identified from the extracellular metabolites of A. terreus were tromethamine,
benzenediol, tetracosamethyl cyclododecasiloxane, hexadecanoic acid, benzoic acid and
pregnatriene. The ethyl acetate extract of the metabolite at 4000 ppm suppressed the growth of M.
phaseolina by 77.78%. The pathway analysis revealed that the metabolites are involved in the
metabolism of drug development, cellular processes, organismal systems and other pathways. The
major identified metabolites and pathways were grouped under biosynthesis of other secondary
metabolites, lipid metabolism and energy metabolism.
Conclusion: The endophytic fungi, A. terreus from mulberry could be an effective antifungal agent
against M. phaseolina. The metabolic pathway analysis found that the major metabolites identified
from A. terreus were also reported in mulberry plants thereby showed the mutual interaction
between the host and endophyte.
