摘要：Dynamic control is a common approach to solve the tradeoff between productivity and yield that exists in engineered microbial metabolisms. Here we explore the possibility of implementing a dynamic control strategy based on direct activity modulation of a hypothetical optogenetic enzyme. With this system we sought to understand whether such a strategy is practical for controlling flux partitioning between biomass and production pathways, and whether it could be used to explore the effect of switching time on the performance of dynamic control strategies. We find that, while a protein-level control system is likely feasible in a model metabolism, several barriers to implementation and performance exist. Based on these limitations we suggest that careful balancing of protein expression at the biomass-production split node is required to implement such a system.
