出版社：Thai Society of Higher Eduction Institutes on Environment
摘要：The activity of ship breaking is one source of iron metal pollution in coastal areas and seawater. This metallic pollution, in high concentrations, was a danger to the local organismsin the habitat. Hence, a means of detoxifying the accumulative harmful molecules of theseheavy metals by bacteria to reduce the toxic level is required. This process is known as thebioremediation of heavy metals. Bacteria was isolated and identifed as originating from theship breaking areas contaminated with iron metal was Burkholderia pseudomallei. This researchaimed at determining the percentage and kinetic rate of Iron (II) removal by Burkholderiapseudomallei. The variables in the research were salinity and pH of medium in laboratory scale.Salinity of medium was used at 15 and 20 ppt. The normal and optimum pH at acidic conditionwas chosen based on the pH effect test. The initial concentration of Iron (II) was 100 mg/L.The Iron (II) removal by Burkholderia pseudomallei was performed for 96 hr. The parametersmeasured were Optical Density (OD), pH, temperature, Iron (II) concentration, and a numberof bacteria colonies. The results showed the iron (II) removal process is optimum at neutral pH.Reactor with pH of 7 showed the percentage of 57.8% and 58.5% at salinity of 15 and 20 ppt,respectively. Salinity affected the kinetics equation of the Iron (II) removal test. The model ofpseudo second order kinetics equation in the reactor with neutral pH and salinity of 20 ppt showedwell-ftted biosorption results (R2 = 0.9668) with a constant kinetics rate (k2) of 0.00038 mg/g.hr.
关键词：Bioremediation; Burkholderia pseudomallei; Heavy metal; Kinetics rate;
pH; Salinity
