With the rapid increase of the number of college students, the limited resources of self-study room has become more and more tense, which result in a series of bad phenomenon like grab seats. Taking a college as an example, this paper investigated the students' self-study room choice behavior by using the method of questionnaire survey. Then the game analysis on self-study room choice behavior of college students was carried out based on systematic thought. As results, the optimization strategy and suggestions about self-study room choice behavior were put forward. The results show that the analysis and improvement to self-study room choice behavior of college students can effectively improve students' self-study room selection efficiency.