摘要：Highlights•Noted exosomal miRNA -93 expression in endometrial cancer patients.•Observed enhanced 1xosomal miRNA-205 expression in cancer patients.•miRNA-205 expression is associated with smoking.AbstractEndometrial Carcinoma (EC) is one of the most common cancers of females’ reproductive system. It has been given first rank in the tumors of obstetrics and gynaecology and day by day the incidence of EC gradually increased in recent years, which has critically harmed the woman health. 100 cases of Endometrial cancer and equal number of female healthy subjects were included and serum were separated from blood sample collected in plain vials from all study participant. Total RNA was extracted from exosomes extracted from serum and cDNA were synthesised from 100 ng of total RNA. cDNA were subjected to quantitative real time PCR to study the microRNA-95 and micro-RNA205 expression among cased and controls using Taqman assay. 3.78 mean fold increased exosomalmicroRNA-95 expression was observed while 0.07 mean fold decreased exosomal microRNA was found in endometrial cancer patients. Increased expression of exosomal miRNA-93 expression were linked with smoking (p = 0.002), grade of tumor (p = 0.01), FIGO stage (p < 0.0001) and distance organ metastases (p < 0.0001). Decreased miRNA-205 (0.07 mean fold) expression of exosomal miRNA-205 was observed in EC patients compared to healthy controls and were found to associated with smoking (p = 0.01), lymph node involvement (p = 0.001), FIGO stage (p = 0.004). Poor overall median survival was observed to be associated with increase exosomalmicroRNA-95 and decreased microRNA-205 of endometrial cancer patients. ROC curves showed exosomal miRNA-93 at cut-off value of 3.25 fold increased in serum based exosomal microRNA-93 expression, sensitivity and specificity were 93% and 97%, respectively (AUC was 0.99, p < 0.0001). A negative correlation was observed between exosomal microRNA-93 and exosomal microRNA-205 expression (r = −0.20, p = 0.04).Study concluded that exosomalmicroRNA-95 and exosomal microRNA-205 was associated with worseness of disease and could be a prognostic indicator for endometrial cancer patients.
