摘要：Graphical abstractDisplay OmittedAbstractWastewater-based epidemiology has become a reputable means to estimate drug consumption within a community. However, these methods typically focus solely on illicit drugs or a single chemical family, with multi-class methods out of favour due to the increased analytical challenges.•A sensitive liquid chromatography – mass spectrometry method was developed for the simultaneous determination of 24 opioids, stimulants and new psychoactive substances in influent wastewater.•Filtered wastewater samples, preserved with sodium metabisulfite, were pretreated and 1000 times concentrated using off-line solid phase extraction.•The method was optimised and fully validated for all compounds, with limits of quantification between 0.2 and 300 ng/L.
