摘要：In order to study the effect of different microbial treatment methods on the removal of petroleum hydrocarbons in soil, microbial remediation of Zichang oil-polluted soil in northern Shaanxi province was studied by means of bacterial inoculation and biological stimulation. By using infrared spectrophotometry to determine the removal effect of different treatment methods on petroleum hydrocarbon, the best scheme for remediation of oil-polluted soil in northern Shaanxi province was determined. In the restoration process, the maximum possible count method (MPN), PCR-agar-electrophoresis, and PCR-DGGE were used to determine the number of oil-hydrocarbon degrading bacteria, catalytic genes, and soil microbial diversity to study the ecological effects of soil microorganisms. The results showed that the remediation effects of different biological treatments on oil-contaminated soil were as follows: biological stimulation (N and P nutrients were added) > biological enhancement (degradation bacteria were added) > and others. There was a positive correlation between the degradation rate of petroleum hydrocarbon in soil and the catalytic gene content of degradable petroleum hydrocarbon. During the restoration process, the number of petroleum hydrocarbons and alkane degrading bacteria in soil was significantly higher than that of PAHs degrading bacteria. The addition of exogenous degrading bacteria sz-1 could significantly improve the diversity of soil bacterial community. The results are helpful to understand the change of microbial ecological effect in bioremediation of petroleum soil.
关键词：Petroleum hydrocarbons;Petroleum contaminated soil;Microbial remediation;Maximum possible count
