摘要：AbstractThe selenium-enriched lotus leaf sclerotium mycelium fermented in 20 L bio-tank was used as raw material to optimize the extraction process of selenium polysaccharide in mycelium and analyze the structure of water-soluble selenium polysaccharide through anti-cancer effect. The optimal extraction conditions of selenium polysaccharide were obtained by experiment and Box-Behnken design method: extraction time was 50 min, extraction temperature was 83 °C, ratio of material to liquid was 1:130 (g:mL), extraction times were 2 times. It is 44.62%, which is 0.02% higher than the predicted value. At this time, the selenium content in the polysaccharide reaches 31.9 μg/g. The purified selenium polysaccharide is analyzed for anticancer effect, which has proliferation inhibition effect on various human cancer cells and inhibits tumor in vivo. Growing.
