摘要：Highlights•Biofilm bacteria and antibiotic sensitivity pattern was studied.•Identified different microbial strains.•P. mirabilis, and Citrobacter were resistant against spectrum of antibiotics.AbstractNosocomial urinary tract infections account for more than 40% of various hospital-acquired infections. Many uropathogens have been reportedly resistant against many commonly available antibiotics, treating patients with UTI infected with catheter associated biofilm producing MDR microorganisms has been a steep challenging task to clinicians. In the present study, a total of 350 clinical samples were collected from catheterized UTI cases admitted in wards and ICU. Specimens were cultured in defined media, and then isolates were biochemically identified. Antibiotics sensitive tests were performed to detect Multidrug resistant (MDR), extensive drug resistance (XDR) and pan drug resistance (PDR) in the isolated organisms. In this study, out of 585 isolates from 350 samples were subjected to biofilm detection. Among this, 63.9% of them were non-biofilm forming organism and 36% of the isolates were found to form biofilm. Out of 211 isolates, significant biofilm producers wereE coli(24%),ESBL E coli(2%),Klebsiella(19%),E. fecalis(8%),S. aureus(3%),P. mirabilis(18%),Pseudomonas aeruginosa(17%) andCitrobacter(9%). In multi drug resistance,E coliwas found to have PAN drug resistance followed byKlebsiellaandPseudomonaswhich exhibited XDR. However all other isolates likeE. fecalis,S. aureus,P. mirabilis, andCitrobacterwere found to be resistant against a limited spectrum of antibiotics. From this study, we could conclude that highest number of isolates was reported from UTI ward than the ICU. Imipenem showed better activity against all isolates.
