摘要：B ursaphelenchusxylophilus(pine wood nematode disease)causes huge damage to pine trees, resulting in huge economic losses. However, its early diagnosis is difficult. At present, the change of microbial community structure has been applied in the early diagnosis of human diseases, but barely on forestry diseases according to reports. Through the analysis of different infection stages in pine wood nematode (PWN), the microbial community structure and its changes, this study preliminarily found out the response law of bacterial flora in pinewood that infected with pine wood nematode disease at early stage. In this study, the P inus mas- soniana(P .m assoniana) were treated with clean water for infection or culture medium carrying PWN, and then the bacteria in P .m assoniana were analyzed by PCR-DGGE and high-throughput sequencing technology. It was found that the difference of bacterial community structure between healthy P .m assoniana and P .m assoniana infected PWN. The conclusion is that no significant difference in bacterial flora type and structure in the group samples of P .m assoniana treated with clear water and blank control group samples. However, the samples o f P .m assoniana inoculated with PWN showed significant differences and regularity in bacterial flora structure before and after onset. For example, K lebsiella and Sphingom onas were positively correlated with the time of inoculation, while A cidocella and P seudoxanthom onas were negatively correlated, which could be used as response monitoring indicators for the infection of pine wood nematode, and could be used to initially judge whether the tree was infected by pine wood nematode and whether corresponding control measures should be taken. At the same time, K lebsiella and Sphingom onas could be further developed and utilized as probiotics for pine disease resistance as considered, which laid a foundation for the development of biological pesticides.
关键词：Pine wilt disease;Bacteria in vivo;P.massoniana;High throughput sequencing
