摘要：A strain CM-11 capable of highly degrading chlorpyrifos-methyl was isolated from soil. The strain CM-11 was identified as Bacillus vallismortis based on 16S rRNA and analysis of morphology, physiological and biochemical characters. The optimal pH value and temperature were 6.8-7.2 and 3035 m. The 6d degradation rate of 100 mg/L chlorpyrifos-methyl by strain CM-11 was at about 99.6% in inorganic salt medium. The strain CM-11 could hydrolyze chlorpyrifos-methyl to 3,5,6-trichloro-2- pyridinol (TCP), but it could only slowly degrade TCP. The combined applications of the strain CM- 11 and the strain TBE-6 was considered an effective means for degrading chlorpyrifos-methyl, because the strain TBE-6 could rapidly degrade TCP. The degradation rate of chlorpyrifos-methyl (100mg/L) by the strain CM-11 and the strain TBE-6 could reach about 100% in 5d. This study may provide theoretic basis and reference for prevention and control of pesticides pollution.
