摘要：The current study was conducted to investigate genetic diversity in local common wheat genotypes collected from different areas of Gilgit Bal- tistan (GB), Pakistan. Landraces was characterized by DNA based markers. The PCR based primer, SSRs (Simple Sequence Repeats) were used for the estimation of genetic distances in 40 selected germplasm. Genetic distances estimated using DNA markers primers ranged from 0 to 100 %. Total 40 accessions were clustered in two main groups A and B comparing 21 and 19 genotypes based on 1,0 data. Accessions number PAK0017512 and PAK0017487 were most distantly related to each other. The principal coordinate analysis (PCoA) based 3D-diagram showed that genotypes PAK0016213, PAK0016214, and PAK0016221 are highly diverged from rest ones and it is suggested that these genotypes should be utilized in further breeding programs aimed at improving common wheat cultivars suitable for high altitude areas. Present results showed presence of enough genetic diversity among hexaploid wheat landraces of GB which can be used in future for developing new improved wheat genotypes.
