期刊名称：Proceedings of the Latvian Academy of Sciences, Section B. Natural, exact, and applied sciences, B dala. Dabaszinatnes
印刷版ISSN：1407-009X
电子版ISSN：1407-009X
出版年度：2019
卷号：73
期号：3
页码：238-243
DOI：10.2478/prolas-2019-0038
语种：English
出版社：The Academy
摘要：AbstractPrunus necrotic ring spot ilarvirus (PNRSV)andApple chlorotic leaf spot trichovirus (ACLSV)are common in plum orchards. The aim of the study was to obtain virus-free planting material ofPrunus domesticaL. by chemotherapyin vitro. Ribavirin at concentrations of 10 to 50 mg·l−1was added to Murashige–Skoog (MS) nutrition medium for virus eradication from microshoots. After a two-week period of chemotherapy, meristems were subcultured monthly on MS medium and proliferation index of shoots was estimated. Microshoots were retested by reverse transcription polymerase chain reaction for presence of virus. At lowest concentrations of 10 mg·l−1ribavirin was entirely ineffective forACLSVand 10 to 30 mg·l−1was ineffective forPNRSVelimination. Ribavirin concentrations of 40 and 50 mg·l−1destroyed both pathogens. However, at higher concentrations of 40 and 50 mg·l−1ribavirin exhibited some signs of phytotoxicity on microshoots in the first sub-cultivation period. In order to test the genetic stability of the microplants after chemotherapy the amplified fragment length polymorphism (AFLP) method was applied. Plant genome stability in ‘Magna Glauca’ at concentrations of 40 mg·l−1was damaged, as the presence of polymorphic AFLP markers were observed.
