摘要：As an indispensable intermediate, androstenedione is widely used in drug manufacturing, especially steroidal drugs. However, the chemical manufacturing process of androstenedione is generally complicated and difficult, and it will cause serious environmental pollution in the production process. The biological method for the production of androstenedione has a very promising development prospect, because it is more economical and environmentally friendly than chemical methods. In order to better produce androstenedione on a large scale, the imbalance between supply and demand can be solved. In this study, the biaqueous phase system was used to increase the substrate concentration, and the method of transforming plant sterol by mycobacterium was used to produce androstenedione. The optimal conditions for the production of androstenedione by microbial assay were determined by orthogonal test: the aqueous two-phase system was water/ sunflower oil, the temperature was 30 °C, the initial pH was 6.5, the substrate concentration was 0.4 g/L, the rotation speed was 250 rpm, and the inoculation was carried out. The amount was 14.83%, the organic ratio was 20.65%, and the liquid loading was 150/500 mL. The preliminary production of androstenedione by microbial method has found suitable process conditions and provided data and theoretical support for its large-scale production.
