摘要：SummaryHuman mononuclear phagocytes comprise several subsets of dendritic cells (DCs), monocytes, and macrophages. Distinguishing one population from another is challenging, especially in inflamed tissues, owing to the promiscuous expression of phenotypic markers. Using a synthetic library of humanized llama single domain antibodies, we identified a novel surface marker for human naturally occurring monocyte-derived DCs. Our antibody targets an extra-cellular domain of LSP-1, specifically on monocyte-derived DCs, but not on other leukocytes, in particular monocytes, macrophages, classical DCs, or the recently described blood DC3 population. Our findings will pave the way for a better characterization of human mononuclear phagocytes in pathological settings.Graphical AbstractDisplay OmittedHighlights•Identification of a humanized llama single chain antibody recognizing surface LSP-1•Surface LSP-1 is a specific marker of monocyte-derived dendritic cells•Classical dendritic cells or blood “DC3” are not stained by the antibody•Surface LSP-1 may be a useful marker for analyzing myeloid cells in human diseaseMolecular Biology
