摘要：SummaryPrecise measurement of action potentials (APs) is needed to observe electrical activity and cellular communication within cardiac tissue. Voltage-sensitive dyes (VSDs) are traditionally used to measure cardiac APs; however, they require acute chemical addition that prevents chronic imaging. Genetically encoded voltage indicators (GEVIs) enable long-term studies of APs without the need of chemical additions, but current GEVIs used in cardiac tissue exhibit poor kinetics and/or low signal to noise (SNR). Here, we demonstrate the use of Archon1, a recently developed GEVI, in hiPSC-derived cardiomyocytes (CMs). When expressed in CMs, Archon1 demonstrated fast kinetics comparable with patch-clamp electrophysiology and high SNR significantly greater than the VSD Di-8-ANEPPS. Additionally, Archon1 enabled monitoring of APs across multiple cells simultaneously in 3D cardiac tissues. These results highlight Archon1's capability to investigate the electrical activity of CMs in a variety of applications and its potential to probe functionally complexin vitromodels, as well asin vivosystems.Graphical AbstractDisplay OmittedHighlights•Genetic sensor Archon1 reports membrane voltage in hiPSC-derived cardiomyocytes•Archon1 monitors action potentials in 2D and 3D cardiac tissue with high sensitivity•Archon1 repeatedly monitored voltage in the same cells and over extended time periods•Voltage dynamics of multiple cells were recorded simultaneously with Archon1Biotechnology; Bioelectronics; Technical Aspects of Cell Biology; Electronic Materials
