摘要：SummaryBoth artificial photosystems and natural photosynthesis have not reached their full potential for the sustainable conversion of solar energy into specific chemicals. A promising approach is hybrid photosynthesis combining efficient, non-toxic, and low-cost abiotic photocatalysts capable of water splitting with metabolically versatile non-photosynthetic microbes. Here, we report the development of a water-splitting enzymatic photocatalyst made of graphitic carbon nitride (g-C3N4) coupled with H2O2-degrading catalase and its utilization for hybrid photosynthesis with the non-photosynthetic bacteriumRalstonia eutrophafor bioplastic production. The g-C3N4-catalase system has an excellent solar-to-hydrogen efficiency of 3.4% with a H2evolution rate up to 55.72 μmol h−1while evolving O2stoichiometrically. The hybrid photosynthesis system built with the water-spitting g-C3N4-catalase photocatalyst doubles the production of the bioplastic polyhydroxybutyrate byR. eutrophafrom CO2and increases it by 1.84-fold from fructose. These results illustrate how synergy between abiotic non-metallic photocatalyst, enzyme, and bacteria can augment solar-to-multicarbon chemical conversion.Graphical AbstractDisplay OmittedHighlights•H2O2-degrading enzymes fromR. eutrophaenable visible-light water splitting by C3N4•C3N4coupled with bovine catalase has a solar-to-hydrogen efficiency of 3.4%•C3N4-catalase increases CO2conversion into bioplastic under light byR. eutropha•Heterotrophic bioplastic production byR. eutrophais also improved by C3N4-catalaseCatalysis; Microbial Biotechnology; Energy Materials
