摘要：SummaryHow the long non-coding RNA (lncRNA) genome in recombinant protein producing Chinese hamster ovary (CHO) cell lines relates to phenotype is not well described. We therefore defined the CHO cell lncRNA transcriptome from cells grown in controlled miniature bioreactors under fed-batch conditions using RNA-Seq to identify lncRNAs and how the expression of these changes throughout growth and between IgG producers. We identify lncRNAs includingAdapt15, linked to ER stress,GAS5, linked to mTOR signaling/growth arrest, andPVT1,linked to Myc expression, which are differentially regulated during fed-batch culture and whose expression correlates to productivity and growth. Changes in (non)-coding RNA expression between the seed train and the equivalent day of fed-batch culture are also reported and compared with existing datasets. Collectively, we present a comprehensive lncRNA CHO cell profiling and identify targets for engineering growth and productivity characteristics of CHO cells.Graphical AbstractDisplay OmittedHighlights•The CHO cell lncRNA transcriptome is defined using RNA-Seq•Correlations between lncRNA expression and CHO cell growth and IgG productivity found•Expression of lncRNAs involved in ER stress correlates to productivity•Expression of lncRNAs involved in mTOR signaling/growth arrest correlates to growthBiological Sciences; Biotechnology; Transcriptomics
